High-speed coherent Raman fingerprint imaging of biological tissues
نویسندگان
چکیده
منابع مشابه
High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues
An imaging platform based on broadband coherent anti-Stokes Raman scattering (BCARS) has been developed which provides an advantageous combination of speed, sensitivity and spectral breadth. The system utilizes a configuration of laser sources that probes the entire biologically-relevant Raman window (500 cm-1 to 3500 cm-1) with high resolution (< 10 cm-1). It strongly and efficiently stimulate...
متن کاملBiological imaging with coherent Raman scattering microscopy: a tutorial.
Coherent Raman scattering (CRS) microscopy is gaining acceptance as a valuable addition to the imaging toolset of biological researchers. Optimal use of this label-free imaging technique benefits from a basic understanding of the physical principles and technical merits of the CRS microscope. This tutorial offers qualitative explanations of the principles behind CRS microscopy and provides info...
متن کاملChemical imaging with frequency modulation coherent anti-Stokes Raman scattering microscopy at the vibrational fingerprint region.
We present a new coherent anti-Stokes Raman scattering (CARS) method that can perform background-free microscopy and microspectroscopy at the vibrational fingerprint region. Chirped broad-band pulses from a single Ti:sapphire laser generate CARS signals over 800-1700 cm(-1) with a spectral resolution of 20 cm(-1). Fast modulation of the time delay between the pump and Stokes pulses coupled with...
متن کاملCoherent backscattering of light from biological tissues.
The angular and temporal distributions of light scattered around the backward direction from biological tissues are measured. An enhancement of scattered light intensity around the backward direction, which is associated with the weak photon localization, is observed. The transport mean free path l(t) and the absorption length l(a) of the light in biological tissue can be obtained from the angu...
متن کاملHigh-speed hyperspectral Raman imaging for label-free compositional microanalysis.
We present high-speed hyperspectral Raman imaging with integrated active-illumination for label-free compositional microanalysis. We show that high-quality Raman spectra can be acquired from as many as ~1,000 spots/sec semi-randomly distributed among a ~100x100 μm(2) area without mechanical scanning. We demonstrate rapid data acquisition from three types of samples: 1) uniform, strong Raman sca...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Photonics
سال: 2014
ISSN: 1749-4885,1749-4893
DOI: 10.1038/nphoton.2014.145